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KMID : 0365819720120010287
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Journal of Pusan Medical College
1972 Volume.12 No. 1 p.287 ~ p.296
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Histochemical Studies on the Staining Properties of the Mucosubstances in the Goblet Cells and Gland Cells of the Trachea
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Abstract
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Comparative histochemical studies were done on the mucosubstances in goblet cells and mucous gland cells of the tracheal mucosa of man, monkey, dog, cat, rabbit, guinea pig, rat, domestic fowl and dove. The mucosubstances were staineI with alcian blue (pH 1.0-2.5), colloidal iron, aldehyde fuchsin, PAS and combined staining of alcian blue - PAS reaction, and those were also stained with the above staining methods after prior treatment of methylation, saponification, methylation-saponification and hyaluronidase digestion. The results obtained were as follows:
1. It was presumes that all mucosubstances in the tracheal goblet cells and mucous gland cells of the human and animal materials contained acid mucosubstances containing sulfated and non sulfated mucin, and neutral mucosubstances.
2. The proportion of acid and neutral mucosubstances was different within each cell types and animals. It was presumed that the mucosubstances of the goblet cells in human materials, monkey, dog, rabbit and guinea pig had nearly equal proportion of both acid and neutral mucosubstances, but those in cat and rat had more neutral mucosubstances and those in fowl and dove had more acid mucosubstances. While the mucoubstances of the mucous gland cells in dog, rabbit, guinea pig and rat had nearly equal proportion of both acid and neutral mucosubstances, but those in human materials, monkey, fowl and dove had more acid mucosubstances.
3. The mucosubstances lost their stainability in alcian blue (pH 2. 5) after methylation but the prior treatment of saponification, methylation-saponification and hyaluronidace digestion had almost no effect on their stainability in alcian blue and alcian blue -PAS reaction.
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